In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity

Viruses

Simrika Thapa²  Mohamed Sarjoon Abdul Cader²  Kalamathy Murugananthan²  Eva Nagy¹  Shayan Sharif¹  Markus Czub²  Mohamed Faizal Abdul-Careem² 

[1] Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada; E-Mails:;Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C64, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada; E-Mails:

关键词: In ovo;    toll-like receptor 21;    CpG-motif containing synthetic DNA;    infectious laryngotracheitis virus;    lung;    chicken;   

DOI  :  10.3390/v7041832

来源: mdpi

【 摘 要 】

Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo delivered CpG DNA significantly reduces ILTV infection pre-hatch correlating with the expression of IL-1β and increase of macrophages in lungs. As assessed in vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-inflammatory mediators. Since we also found that in ovo CpG DNA delivery maintains increased macrophages in the lungs post-hatch, we infected the chickens on the day of hatch with ILTV. We found that in ovo delivered CpG DNA significantly reduces mortality and morbidity resulting from ILTV infection encountered post-hatch. Thus, CpG DNA can be a candidate innate immune stimulant worthy of further investigation for the control of ILTV infection in chickens.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.