Effects of phosphorus on polyphosphate accumulation by Cunninghamella elegans

Brazilian Journal of Microbiology

Marcos Antonio Barbosa De Lima²  Aline Elesbão Do Nascimento²  Wanderley De Souza¹  Kazutaka Fukushima¹  Galba Maria De Campos-takaki² 

[1] ,Universidade Católica de Pernambuco Departamentos de Biologia e Química Núcleo de Pesquisas em Ciências AmbientaisRecife PE ,Brasil

关键词: polyphosphate;    accumulation;    ultrastructural cytochemistry;    Cunninghamella elegans;    ortophosfate;    polifosfato;    acumulação;    citoquímica ultraestrutural;    Cunninghamella elegans;    ortofosfato;   

DOI  :  10.1590/S1517-83822003000400016

来源: SciELO

【 摘 要 】

The content of inorganic polyphosphate and the polymeric degree of these compounds were evaluated during the growth of Cunninghamella elegans in medium containing varying orthophosphate (Pi) concentrations. For this purpose, a combination of chemical methods for polyphosphate extraction and ultrastructural cytochemistry were used. The orthophosphate and glucose consumption was also determined during the fungal cultivation. At Pi concentrations of 0.5, 2.5 and 0.0 g/L, the maximum amounts of biomass were 3.18, 3.29 and 0.24 g/L, respectively. During growth the cells accumulated Pi from the medium. At three days of growth the biomass consumed up to 100 and 95% of Pi from the media at initial concentrations of 0.5 and 2.5 g/L, respectively. Polyphosphate was observed at different Pi concentrations in medium and at different stages of growth. Polyphosphate was assayed by the content of labile phosphorus in water, acid-insoluble and alkali-soluble fractions. The content of fractions changed according to phosphorus concentration in the media and growth phase. During growth on all three media used, the cytochecmical behavior of polyphosphate changed considerably. The results obtained in this study reveal a potential of Cunninghamella elegans in the polyphosphate accumulation, and suggest a future application in the biotechnological processes.

【 授权许可】

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