Leptospira spp detection by Polymerase Chain Reaction (PCR) in clinical samples of captive black-capped Capuchin monkey (Cebus apella)

Brazilian Journal of Microbiology

Eliana Scarcelli¹  Rosa Maria Piatti¹  José Daniel Luzes Fedullo¹  Faiçal Simon¹  Maristela Vasconcellos Cardoso¹  Vanessa Castro¹  Simone Miyashiro¹  Margareth Élide Genovez¹ 

[1] ,Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal São Paulo SP ,Brasil

关键词: Leptospirosis;    nonhuman primate;    PCR;    Cebus apella;    Leptospirose;    primata não-humano;    PCR;    Cebus apella;   

DOI  :  10.1590/S1517-83822003000200010

来源: SciELO

【 摘 要 】

Leptospirosis is a widely distributed zoonosis that affects domestic and wild animals, and that has the man as the end point of its epidemiological chain. Leptospirosis diagnosis in primates is more difficult than in other animal species, as clinical signs and lesions are less evident and antibody response is detected only for short periods. The aim of this article was to describe the detection of Leptospira spp using polymerase chain reaction (PCR), in clinical samples from one captive black-capped Capuchin monkey (Cebus apella), which presented characteristics compatible with leptospirosis (jaundice and haemorrhagic kdney) in the macroscopic post-mortem examination. A friable kidney fragment and urine sample were cultured and submitted to experimental inoculation in guinea pigs and PCR using genus specific primer pair targeting the 16S rRNA region from Leptospira interrogans serovar canicola. Isolation of the agent was negative both in culture and experimental inoculation. The PCR amplification of the clinical samples showed a 330 pb amplified fragment that corresponds to the Leptospira genus. Based on these results PCR was considered an important tool for leptospira detection in nonhumam primates, more sensitive and specific than other techniques, especially considering that the viability of the pathogen was not possible. These advantages enable the detection of the leptospiras in urine and kidney, even when autolysed, frozen or badly conserved, which prevented the isolation and experimental inoculation from positive results.

【 授权许可】

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