Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil

Pesquisa Veterinária Brasileira

Ana Lys Bezerra Barrradas Mineiro²  Rômulo José Vieira²  Érica Azevedo Costa¹  Renato Lima Santos¹  Larissa Maria Feitosa Gonçalves²  Sônia Maria Carvalho²  Maria Rosa Quaresma Bomfim¹  Francisco Assis Lima Costa² 

[1] ,Universidade Federal do Piauí Centro de Ciências Agrárias Setor de Patologia AnimalTeresina PI ,Brazil

关键词: Leptospirosis;    immunohistochemistry;    PCR;    cattle;    Leptospirose;    imuno-histoquímica;    PCR;    bovino;   

DOI  :  10.1590/S0100-736X2011001000005

来源: SciELO

【 摘 要 】

The presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue

【 授权许可】

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