Template preparation for rapid PCR in Colletotrichum lindemuthianum

Brazilian Journal of Microbiology

M. Gabriela Roca²  Lisete C. Davide²  Alan E. Wheals¹ 

[1] ,Universidade Federal de Lavras Departamento de Biologia Lavras MG ,Brasil

关键词: anthracnose;    hygromycin resistance;    internal transcribed spacer;    rapid screening;    PCR methodology;    antracnose;    espaços transcritos internamente;    metodolologias de PCR;    resistência a higromicina;    triagens rápidas;   

DOI  :  10.1590/S1517-83822003000100003

来源: SciELO

【 摘 要 】

Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).

【 授权许可】

CC BY-NC   
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