Time course of virus-like particles (VLPs) double-stranded rna accumulation in toxigenic and non-toxigenic strains of Aspergillus flavus

Brazilian Journal of Microbiology

Valéria N. Silva²  Edison Luiz Durigon²  Maria De Fátima Costa Pires¹  Alexandre Lourenço²  Maria Jacinta De Faria²  Benedito Corrêa² 

[1] ,Universidade de São Paulo Instituto de Ciências Biomédias Departamento de MicrobiologiaSão Paulo SP ,Brasil

关键词: mycovirus;    double-stranded RNA;    virus-like particles;    Aspergillus flavus;    micovírus;    RNA de fita dupla;    partículas semelhantes a vírus;    Aspergillus flavus;   

DOI  :  10.1590/S1517-83822001000100013

来源: SciELO

【 摘 要 】

Two strains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication.

【 授权许可】

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