Isolation and characterization of aflatoxigenic Aspergillus spp. from maize of livestock feed from Bogor

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Sukmawati, D.^1 ; Setyaningsih, A.^1 ; Handayanik, T.^1 ; Rahayu, S.^1 ; Rustam, Y.^1 ; Moersilah, M.^2 ; Wahyudi, P.^3 ; Husna, S.N.A.^4

Department of Biology, Faculty of Mathematics and Sciences, State University of Jakarta, Jakarta, Indonesia^1

Department of Chemistry, Faculty of Mathematics and Sciences, State University of Jakarta, Jakarta, Indonesia^2

BPPT, Jakarta, Indonesia^3

Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Indonesia^4

关键词: Aspergillus flavus;    Aspergillus spp;    Blue fluorescence;    Direct method;    Isolation and characterization;    Livestock feed;    Molecular identification;    Selective medium;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012105/pdf
DOI  :  10.1088/1757-899X/434/1/012105

来源: IOP

【 摘 要 】

Aflatoxin is a naturally mutagenic and carcinogenic mycotoxin found in feed and food. Aflatoxin contamination on maize can affect productivity of feed and food manufacture. The purpose of this study was to obtain isolates and to understand the characteristics of aflatoxigenic Aspergillus flavus from maize on livestock feed. The study was divided into two stages: isolation and molecular identification of fungal ITS rDNA region in livestock feed obtained from Bogor, West Java. Isolation was conducted by enrichment and direct method using Dichloran-Glycerol (DG18) medium, while aflatoxin test and fungal characterization were done by CAM (Coconut Agar Medium) and selective medium of Aspergillus flavus and parasiticus (AFPA), respectively. The result showed that 9 of isolates are identified as molds (P2, P3, P4, P5, P7, P8, are green sporulated, while P1, P6, P9 are black sporulated). Aflatoxin detection on P3 and P8 isolates did not produce blue fluorescence fluid in CAM and did not form a beige ring on the back of petri. This indicated P3 and P8 did not produce aflatoxin on CAM media. Molecular identification results that P3 and P8 isolates have 100% and 99% homology with A. flavus, respectively.