Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation

Brazilian Archives of Biology and Technology

Raji Rahulan¹  G Manjunath¹  Kesavan Madhavan Nampoothiri¹  Ashok Pandey¹ 

[1] ,National Institute for Interdisciplinary Science and Technology; Biotechnology Division Kerala,India

关键词: Streptomyces gedanensis;    Methionine amino peptidase;    Fermentation;    Box Behnken design;   

DOI  :  10.1590/S1516-89132014000200005

来源: SciELO

【 摘 要 】

A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.

【 授权许可】

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