Cloning of Ammopiptanthus mongolicus C-repeat-binding factor gene and its cold-induced tolerance in transgenic tobacco

Brazilian Archives of Biology and Technology

Lijiang Gu¹  Han Zhang¹  Huiming Guo¹  Hongmei Cheng¹ 

[1] ,Chinese Academy of Agricultural Sciences Biotechnology Research Institute Beij,PR China

关键词: Ammopiptanthus mongolicus;    C-repeat-binding factor;    Gene transformation;    Physiological index detection;   

DOI  :  10.1590/S1516-89132013005000005

来源: SciELO

【 摘 要 】

C-repeat-binding factors (CBFs) are a type of important regulon in stress-related signal transduction pathways that control plant tolerance of abiotic stress. Ammopiptanthus mongolicus is the only evergreen broadleaf shrub in the northwest desert of China. The species shows strong resistance to environmental stress, especially to cold stress. An A. mongolicus CBF1 gene (AmCBF1) was cloned and transformed into tobacco. Expression of AmCBF1 could be detected in A. mongolicus shortly after exposure to low temperature of 4°C. Analysis on ratio of electrolytic leakage, soluble sugar content, free proline content, malondialdehyde (MDA) content and peroxidase (POD) activity before and after cold treatment (4°C) for 24 h indicated AmCBF1 conferred higher cold tolerance to AmCBF1 transgenic tobacco compared with the wild type and empty vector transformed tobacco.

【 授权许可】

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