The Effects of Carbenicillin on Growth and Differentiation of Three Berries

4th International Conference on Advances in Energy Resources and Environment Engineering

能源学;生态环境科学

Song, Ke^1 ; Wang, Wei^1 ; Zhang, Ziqi^1 ; Yang, Jinhui^1 ; Wu, Nan^2 ; Li, Bing^3

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin, China^1

College of Mechanical and Electrical Engineering, Tianjin Agricultural University, Tianjin, China^2

Tianshi University, Tianjin, China^3

关键词: Agrobacterium mediated transformations;    Agrobacterium tumefaciens;    Carbenicillin;    Gene transformation;    Inhibitive effects;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/237/5/052011/pdf
DOI  :  10.1088/1755-1315/237/5/052011

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The research aimed to provide references for further study of gene transformation of blueberry, raspberry and cranberry in Agrobacterium-mediated transformation. Effects of carbenicillin on the growth and differentiation of various explants of three berries were studied, and the inhibitive effects of carbenicillin upon Agrobacterium tumefaciens also were studied. The results showed that the optimum carbenicillinon's concentration was 200∼ 300 mg/kg in the differentiating stage of blueberry and raspberry, and the time of subculture was 7 days; the optimum carbenicillinon's concentration was 400 mg/kg in the differentiating stage of cranberry, and the time of subculture was 7 ∼ 14 days; The optimum carbenicillin's concentration was under 200 mg/kg in the rooting stage of three berries.