Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Brazilian Archives of Biology and Technology

Qiaoyan Zhang¹  Junqing Qian¹  Lingzhi Ma¹ 

[1] ,Zhejiang University of Technology College of Pharmaceutical Science Hangzhou Zhejiang ,China

关键词: Supercritical CO2;    Mutation;    pH Drop;    Lipase;    Flavobacterium sp;   

DOI  :  10.1590/S1516-89132013000400003

来源: SciELO

【 摘 要 】

This work aimed to obtain an ideal mutant strain with higher lipase yield using hydrazine hydrate (HZH) as a novel additive to treat Flavobacterium sp. strain YY25 by supercritical CO2. The survival rate and the positive mutation rate of the tested strain were strongly dependent on the dose of HZH. The treatment by 0.5% HZH in supercritical CO2 (8 MPa, 35°C) for 30 min provided 58.3% of positive mutation rate and an expected mutant strain with about 76.7% increase in lipase yield compared with the wild strain. Possible mutagenesis mechanisms were further explored. The analysis on pH drop of the treated seed liquid was performed to better understand the interaction. Results proved that the induced mutation with enhanced yield of lipase could be achieved by co-mutagenesis of supercritical CO2 and HZH.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License