A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

Brazilian Archives of Biology and Technology

Francisca Acevedo²  Leticia Pizzul¹  María Del Pilar Castillo¹  Olga Rubilar¹  María Elena Lienqueo¹  Gonzalo Tortella¹  María Cristina Diez¹ 

[1] ,Universidad de La FronteraTemuco,Chile

关键词: white-rot fungi;    ligninolytic enzymes;    purification;    lignocellulosic material;   

DOI  :  10.1590/S1516-89132011000600013

来源: SciELO

【 摘 要 】

In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.

【 授权许可】

CC BY-NC   
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