Brazilian Archives of Biology and Technology | |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes | |
Barnita Ghosh1  Rina Rani Ray1  | |
[1] ,Presidency University Department of Zoology ,India | |
关键词: Isoamylase; Rhizopus oryzae; SSF; waste utilization; | |
DOI : 10.1590/S1516-89132011000500003 | |
来源: SciELO | |
【 摘 要 】
Extra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high amount of isoamylase within a relatively very short period and the capability of degrading wastes could make the strain suitable for commercial production of the enzyme.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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