Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes

Brazilian Archives of Biology and Technology

Barnita Ghosh¹  Rina Rani Ray¹ 

[1] ,Presidency University Department of Zoology ,India

关键词: Isoamylase;    Rhizopus oryzae;    SSF;    waste utilization;   

DOI  :  10.1590/S1516-89132011000500003

来源: SciELO

【 摘 要 】

Extra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high amount of isoamylase within a relatively very short period and the capability of degrading wastes could make the strain suitable for commercial production of the enzyme.

【 授权许可】

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