Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate

Brazilian Archives of Biology and Technology

Patricia Braunig¹  Rafael Diego Da Rosa¹  Caroline Heidrich Seibert¹  Mariana Borsa¹  Patricia Hermes Stoco¹  Edmundo Carlos Grisard¹  Aguinaldo Roberto Pinto¹ 

[1],Universidade Federal de Santa Catarina Departamento de Microbiologia, Imunologia e Parasitologia Florianópolis SC ,Brasil

关键词: WSSV;    VP28;    recombinant protein;    penaeid shrimp;   

DOI  :  10.1590/S1516-89132011000200023

来源: SciELO

【 摘 要 】

In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil.

【 授权许可】

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