Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes

Diseases of Aquatic Organisms

Yan Liang Jie Huang¹  Xiao-Ling Song Pei-Jun Zhang Huai-Su Xu¹ 

关键词: Viral attachment protein;    White spot syndrome virus;    WSSV;    Shrimp;    VP28;   

DOI  :  10.3354/dao066081

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: White spot syndrome virus (WSSV) is a major shrimp pathogen that has a widespread negative affect on shrimp production in Asia and the Americas. It is known that WSSV infects shrimp cells through viral attachment proteins (VAP) that bind with shrimp cell receptors. However, the identity of both WSSV VAP and shrimp cell receptors remains unclear. We used digoxigenin (DIG)-labeled shrimp hemocyte and gill cell membranes to bind to WSSV proteins immobilized on nitrocellulose membranes, and 4 putative WSSV VAP (37 kDa, 39 kDa and 2 above 97 kDa) were identified. Mass spectrometric analysis identified the 37 kDa putative VAP as the product of WSSV gene VP281.

【 授权许可】

Unknown