Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium

Brazilian Archives of Biology and Technology

Muthusamy Ashok Kumar²  Kanapathi Thangavel Kasirajan Anandapandian¹  Karuppiah Parthiban¹ 

[1] ,Karunya University Department of Biotechnology Coimbatore,India

关键词: Biofilm;    Marine Bacteria;    EPS;    SEM;    TLC and FTIR;   

DOI  :  10.1590/S1516-89132011000200006

来源: SciELO

【 摘 要 】

Marine water was used for the biofilm forming bacterial isolation. A biofilm forming device was made by using PVC pipes with sterile glass slides. Five morphologically different bacteria were isolated from the scrapping of glass slides from the device after a month period of time. The efficiency of EPS production was checked for all the isolates. Using scanning electron microscope (SEM), the morphology of microbial cells and colonies was studied. The maximum EPS was produced by the strain B3, which was analyzed and characterized by thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) spectra. B3 EPS displayed a strong absorption band of - OH at 3415.31 cm-1 and COOH at 1631.48 cm-1 showing it to be polysaccharide.

【 授权许可】

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