The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS

Brazilian Archives of Biology and Technology

Juliana Ferrari Ferreira²  José Carlos Curvelo Santana¹  Elias Basile Tambourgi² 

[1] ,Universidade Estadual de Campinas Faculdade de Engenharia Química Departamento de Engenharia de Sistemas QuímicosCampinas SP ,Brasil

关键词: bromelain;    aqueous two-phase systems;    partitioning;    characterization;    pineapple;   

DOI  :  10.1590/S1516-89132011000100017

来源: SciELO

【 摘 要 】

In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain was purified. Optimum conditions of bromelain use were found at pH 7 and between 30-40ºC.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License