Brazilian Archives of Biology and Technology | |
The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS | |
Juliana Ferrari Ferreira2  José Carlos Curvelo Santana1  Elias Basile Tambourgi2  | |
[1] ,Universidade Estadual de Campinas Faculdade de Engenharia Química Departamento de Engenharia de Sistemas QuímicosCampinas SP ,Brasil | |
关键词: bromelain; aqueous two-phase systems; partitioning; characterization; pineapple; | |
DOI : 10.1590/S1516-89132011000100017 | |
来源: SciELO | |
【 摘 要 】
In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain was purified. Optimum conditions of bromelain use were found at pH 7 and between 30-40ºC.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202005130166312ZK.pdf | 250KB | download |