Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis

Brazilian Archives of Biology and Technology

Maria Giovana Binder Pagnoncelli²  Nathália Kelly De Araújo²  Nayane Macêdo Portela Da Silva²  Cristiane Fernandes De Assis²  Sueli Rodrigues¹  Gorete Ribeiro De Macedo² 

[1] ,Universidade Federal do Rio Grande do Norte Departamento de Engenharia Química Laboratório de Engenharia BioquímicaNatal RN ,Brasil

关键词: Chitosanase;    Paenibacillus ehimensis;    chitosanase production;    crude enzymes and TLC;   

DOI  :  10.1590/S1516-89132010000600023

来源: SciELO

【 摘 要 】

The chitosanase production by Paenibacillus ehimensis was studied in submerged cultures and the chitosan hydrolysis was evaluated by using these enzymes without purification. The bacterium produced inducibles enzymes after 12 h of growth in a culture medium containing 0.2% (w/v) of soluble chitosan as carbon source. The enzyme production was strongly repressed by the presence of glucose. The production started as soon as the available sugars finished in the culture medium. The maximum level of chitosanase activity was 500 U.L-1 at 36°C after 36 h incubation. The crude enzyme was optimally active at pH 6.0 and 55°C and in these conditions, the enzyme presented good stability (6 days). The enzyme without purification was used to hydrolyze the chitosan which resulted chitooligosaccharides between 20 and 30 min of reaction.

【 授权许可】

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