Amplification of the cap20 pathogenicity gene and genetic characterization using different markers molecular in Colletotrichum gloeosporioides isolates

Brazilian Archives of Biology and Technology

Danielli Barreto Maciel²  Lílian Vieira De Medeiros²  Vivian Vieira De Medeiros²  Mariele Porto Carneiro Leão²  Luis Eduardo Aranha Camargo¹  Neiva Tinti De Oliveira² 

[1] ,Universidade Federal de Pernambuco Departamento de Micologia Pernambuco PE ,Brasil

关键词: cap20 gene;    Colletotrichum gloeosporioides;    pathogenicity;   

DOI  :  10.1590/S1516-89132010000600001

来源: SciELO

【 摘 要 】

Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative to separate the Colletotrichum sp, and that primer EI1 demonstrated greater genetic diversity. The amplification of the DNA of the Colletotrichum isolates to the cap20 gene with primers P1 and P2 indicated that this gene could present variations into C. gloeosporioides related with the host, and also that it was present in other Colletotrichum sp.

【 授权许可】

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