Light microscope observation of circulating human lymphocytes cultured in vitro

Brazilian Archives of Biology and Technology

Naila Francis Paulo De Oliveira¹  Mary Anne Heidi Dolder¹  Selma Candelária Genari¹ 

[1] ,Universidade Estadual de Campinas Instituto de Biologia Departamento de Biologia CelularCampinas SP ,Brasil

关键词: blood;    in vitro;    lymphocytes;    microscopy;    morphology;    Percoll;   

DOI  :  10.1590/S1516-89132010000500013

来源: SciELO

【 摘 要 】

The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.

【 授权许可】

CC BY-NC   
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