Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus

Brazilian Archives of Biology and Technology

Marcia Luciana Cazetta²  Rubens Monti¹  Jonas Contiero² 

[1] ,Universidade Estadual Paulista Instituto de Biociências de Rio Claro Departamento de Bioquímica e MicrobiologiaRio Claro SP ,Brasil

关键词: Kluyveromyces marxianus var. bulgaricus;    sucrose;    inulinase;    biomass;    batch fermentation;   

DOI  :  10.1590/S1516-89132010000300027

来源: SciELO

【 摘 要 】

The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License