Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14

Brazilian Archives of Biology and Technology

André Lacerda Ulysses De Carvalho²  Fábio Henrique Portella Corrêa De Oliveira²  Rosa De Lima Ramos Mariano¹  Ester Ribeiro Gouveia²  Ana Maria Souto-maior² 

[1] ,Universidade Federal de Pernambuco Departamento de Antibióticos Recife PE ,Brasil

关键词: Bacillus subtilis;    batch cultivation;    oxygen availability;    antimicrobial activity;   

DOI  :  10.1590/S1516-89132010000300020

来源: SciELO

【 摘 要 】

The physiology of B. subtilis R14 was investigated in minimal medium under excess-oxygen and oxygen-limited conditions. Growth and efficient sporulation could be achieved in excess-oxygen culture on medium with readily metabolizable carbon and nitrogen sources, which allowed high growth rate and high biomass yield. A short transition phase between the exponential growth and sporulation could be attained by formulating a medium with a well-balanced C/N ratio. Under oxygen-limitation, but in the presence of essential nutrients (i.e. excess-nutrient cultivation), B. subtilis R14 produced bioactive compounds, which showed activity against several phytopathogenic bacteria. Under anaerobic condition, the organism did not grow neither through fermentation nor anaerobic respiration. However, addition of pyruvate to the medium allowed its growth through fermentation and anaerobic respiration. The knowledge acquired in this work could be relevant both for the design of a production process as well as for the formulation of an effective commercial biocontrol product.

【 授权许可】

CC BY-NC   
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