Genotype-by-Environment-by-Environment Interactions in the Saccharomyces cerevisiae Transcriptomic Response to Alcohols and Anaerobiosis

G3: Genes, Genomes, Genetics

article

Maria Sardi¹  Molly Krause¹  Justin Heilberger¹  Audrey P. Gasch¹ 

[1] Great Lakes Bioenergy Research Center,University of Wisconsin-Madison, Madison WI,53706;Laboratory of Genetics, University of Wisconsin-Madison, Madison WI,53706

关键词: Key words: yeast;    gene expression;    stress response;    alcohol;    oxygen availability;   

DOI  :  10.1534/g3.118.200677

学科分类：社会科学、人文和艺术（综合）

来源: Genetics Society of America

【 摘 要 】

Next generation biofuels including longer-chain alcohols such as butanol are attractive as renewable, high-energy fuels. A barrier to microbial production of butanols is the increased toxicity compared to ethanol; however, the cellular targets and microbial defense mechanisms remain poorly understood, especially under anaerobic conditions used frequently in industry. Here we took a comparative approach to understand the response of Saccharomyces cerevisiae to 1-butanol, isobutanol, or ethanol, across three genetic backgrounds of varying tolerance in aerobic and anaerobic conditions. We find that strains have different growth properties and alcohol tolerances with and without oxygen availability, as well as unique and common responses to each of the three alcohols. Our results provide evidence for strain-by-alcohol-by-oxygen interactions that moderate how cells respond to alcohol stress.

【 授权许可】

CC BY|CC BY-NC