New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil

Brazilian Archives of Biology and Technology

Dicezar Gonçalves²  Jane Eire Gabriel¹  Humberto Maciel França Madeira¹  Guilherme Schnell E Schühli¹  Vânia Aparecida Vicente¹ 

[1] ,Universidade Federal do Paraná Divisão de Bioprocessos e Biotecnologia Curitiba PR ,Brasil

关键词: Staphylococcus aureus;    molecular markers;    subclinical mastitis;    clinical mastitis;    mastitis detection;   

DOI  :  10.1590/S1516-89132010000200014

来源: SciELO

【 摘 要 】

The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for effective and early diagnostic tool for S. aureus infection in dairy herds is suggested.

【 授权许可】

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