Molecular cloning of αRYR hotspot region 1 from broiler chicken

Brazilian Archives of Biology and Technology

Iris Lamberti Ziober²  Fernanda Gonzales Paião²  Silvana Regina Rockenbach Marin¹  Denis Fabricio Marchi²  Eliseu Binneck¹  Alexandre Lima Nepomuceno¹  Luiz Lehmann Coutinho¹  Massami Shimokomaki² 

[1] ,Universidade Estadual de Londrina Centro de Ciências Agrárias Departamento de Ciência e Tecnologia de AlimentosLondrina PR ,Brasil

关键词: αRYR;    PSE;    chicken;   

DOI  :  10.1590/S1516-89132009000700029

来源: SciELO

【 摘 要 】

Samples of Pectoralis major m. were collected, and an RT-PCR analysis of the a-Ryanodine receptor (a RYR) from chicken mRNA hotspot region spanning aminoacid residues 386 to 540, numbered according to the turkey sequence, revealed two classes of transcripts. The sequences of the first class were similar to turkey and human with 97% and 74% of identity, respectively, and included all transcripts with substitutions in the nucleotide sequence. The second class was characterized by the deletion of nucleotides, leading to a premature stop codon and coding for a truncated and nonfunctional protein. These results are to date the first report related to the sequencing of the chicken αRYR hotspot region 1, which will possibility serve as a guide for further studies regarding a solution in the poultry production chain related to the problem of pale, soft and exudative (PSE) meat.

【 授权许可】

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