A bovine teat papilloma specimen harboring Deltapapillomavirus (BPV-1) and Xipapillomavirus (BPV-6) representatives

Brazilian Archives of Biology and Technology

Marlise Pompeo Claus²  Michele Lunardi²  Amauri Alcindo Alfieri²  Rodrigo Alejandro Arellano Otonel²  Lara Munique Ferracin¹  Maria Helena Pelegrinelli Fungaro¹  Alice Fernandes Alfieri² 

[1] ,Universidade Estadual de Londrina Laboratório de Virologia Animal Departamento de Medicina Veterinária PreventivaLondrina PR ,Brasil

关键词: cattle;    cutaneous papillomatosis;    BPV;    co-infection;   

DOI  :  10.1590/S1516-89132009000700012

来源: SciELO

【 摘 要 】

The common occurrence of multiple papillomavirus infections has been shown in several studies involving the human host. However, investigations with the aim of identifying mixed papillomavirus infections in cattle have been conducted only recently. In the current work we describe a co-infection with two different bovine papillomavirus (BPV) types that was identified in a bovine teat papilloma. The skin wart was obtained from a cow belonging to a Brazilian beef herd. A PCR assay was carried out with the FAP primer pair, which amplifies a partial segment of the L1 gene (approximately 478 bp), and the amplicon was submitted to direct sequencing. Because nucleotide sequences with satisfactory quality scores were not obtained, the amplicon was cloned and further sequencing, involving ten selected clones, was performed. The sequence analysis of the cloned inserts revealed the presence of two different BPV types. BPV-1 (Deltapapillomavirus genus) was detected in six clones, while BPV-6 (Xipapillomavirus genus) was detected in four clones. This finding confirms the presence of BPV co-infection associated with cutaneous papillomatosis in cattle.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License