Utilization of the biorreactor of imersion by bubbles at the micropropagation of Ananas comosus L. Merril

Brazilian Archives of Biology and Technology

Gessiel Newton Scheidt²  Andréa Haruko Arakaki²  Jacqueline Sugitani Chimilovski²  Augustus Caeser Franke Portella²  Michele Rigon Spier²  Adenise Lorenci Woiciechowski²  Luiz Antonio Biasi¹  Carlos Ricardo Soccol² 

[1] ,Universidade Federal do Paraná Departamento de Bioprocessos e Biotecnologia Curitiba PR ,Brasil

关键词: Bioreactors;    Ananas comosus;    micro propagation;   

DOI  :  10.1590/S1516-89132009000700005

来源: SciELO

【 摘 要 】

The research for new techniques of in vitro cultivation is being object of many studies around the world, in order to optimize and decrease production costs of seedlings with agronomical interest. The main goal of this work was to compare different systems of in vitro cultivations using Ananas comosus L. Merril. So, the in vitro growth of the plantlets was promoted in two different bioreactors: Bioreactor of Immersion by Bubbles (B.I.B.®) and the Reactor of Temporary Immersion (R.I.T.A.®) with immersion cycle every 2 hours for 15 minutes and the traditional system in flasks with 200 mL. All cultivation systems used the MS liquid nutritive solution, supplemented with BAP (1 mgL-1), ANA (0.25 mgL-1), sucrose (30 gL-1) and Tween 20® (0.5 µL). The pH was adjusted to 5.8 and sterilized at 120°C for 15 minutes. The cultures were kept into a growth room during 30 days, with controlled temperature of 25±2°C, under white cold light (46.8 µmol.m-2.s-1), with photoperiod of 16 hours. The experimental design used was randomized, with three treatments, three repetitions and ten plants each stage. Among the evaluated systems, the BIB® presented the best results for the tested variables, mainly the total number of shoots, being 2.3 e 3.1 times superior when compared with the system R.I.T.A.® and the traditional consecutively. So the system of immersion by bubbles turns into an effective equipment to produce seedlings of pineapple in large scale.

【 授权许可】

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