Micropropagation of Etlingera elatior (Zingiberaceae) by using axillary bud explants

Dynamic Chiropractic

A. H. A. Abdelmageed¹  F. M. A. Norhana²  Q. Z. Faridah³  Midhzar Abdul Kadir⁴  A. A. Julia⁵ 

[1] Biodiversity Unit, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.;Department of Agriculture Technology, Faculty of Agriculture, Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.;Department of Horticulture, University of Khartoum, Shambat 13314, Khartoum North, Sudan.;Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

关键词: Etlingera elatior;    axillary bud;    medicinal plant;    micro propagation;    plant growth regulator;    Zingiberaceae.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Axillary bud explants ofEtingera elatiorwerecultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of cytokinin (BAP) or auxin (IAA) as plant growth regulators. The addition of 22.2 µM BAP to the MS medium produced the highest mean number of shoots (3.67) per explants as compared to other concentrations. The best shoots length (4.20 cm) was obtained from the medium containing 26.6 µM of BAP. Forin vitrorooting of shoot, MS medium supplemented with 11.4 µM IAA produced the highest number of root, whereas 34.2 µM IAA gave the longest roots in the medium. The proliferated shoots were green and healthy in appearance. The Plantlets with well developed roots were hardened, acclimatized and planted out successfully with a survival rate of 75%.

【 授权许可】

Unknown