Batch and continuous extraction of bromelain enzyme by reversed micelles

Brazilian Archives of Biology and Technology

Ana Maria Frattini Fileti¹  Gilvan Anderson Fischer¹  José Carlos Curvelo Santana¹  Elias Basile Tambourgi¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Engenharia Química Campinas SP ,Brasil

关键词: reversed micelles;    liquid-liquid extraction;    bromelain;    pineapple;    optimization;    surface response;   

DOI  :  10.1590/S1516-89132009000500021

来源: SciELO

【 摘 要 】

The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License