Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m

Brazilian Archives of Biology and Technology

Silvana Ramos Farias Moreno²  Jorge José De Carvalho¹  Ana Lúcia Nascimento¹  Beni Olej²  Emely Kazan Rocha¹  Adriano Arnobio²  Mário Bernardo-filho¹  Luiz Querino De Araújo Caldas²  Hayden Honeycut¹ 

[1],Universidade Federal FluminenseNiterói RJ ,Brasil

关键词: Uncaria tomentosa;    technetium-99m;    radiolabeling;    blood constituents;   

DOI  :  10.1590/S1516-89132008000700024

来源: SciELO

【 摘 要 】

The influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p<0.05) the labeling of blood constituents by in vitro methods. These effects could be due to chelation of stannous and /or pertechnetate ions and blockage of the Tc-99m bindings sites.

【 授权许可】

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