Influence of the 15-HETE on cytosolic [ Ca2+] i of the rabbit pulmonary artery smooth muscle cells

Brazilian Archives of Biology and Technology

Xiaobo Tang¹  Rong Zhang¹  Zhigang Wang¹  Lei Yu¹  Shanshan Li¹  Daling Zhu¹ 

[1] ,Harbin Medical University College of Pharmacy ,China

关键词: 15-hydroxyeicosatetraenoic acid;    hypoxia;    pulmonary artery rings;    pulmonary artery smooth muscle cells;    [ Ca2+]i;   

DOI  :  10.1590/S1516-89132008000200007

来源: SciELO

【 摘 要 】

Extracellular Ca2+ influx was blocked by L-type Ca2+ channel blocker nifedipine to observe the effects of 15-hydroxyeicosatetraenoic acid on the constriction of rabbit pulmonary artery rings and on the changes of Ca2+ level in the rabbit pulmonary artery smooth muscle cells, and further to investigate the mechanism of the calcium mobilization induced by the 15-HETE under hypoxic conditions. The effect of extracellular Ca2+ on tension of the rabbit PA rings was also studied. Nifedipine (10 µ mol/L) had no effect on 1 µ mol/L 15-hydroxyeicosatetraenoic acid induced vasoconstriction under normoxic and hypoxic conditions. Intracellular Ca2+ increased markedly in the 15-HETE group (cells were exposed to 1 µ mol/L 15-HETE for 8 min during culture) compared to the control group (P < 0.05). The study demonstrated that the 15-HETE could induce the elevation of Ca2+ in the pulmonary artery smooth muscle cells and the elevated calcium came from the release of the intracellular calcium.

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