Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge

Brazilian Archives of Biology and Technology

Leila Larisa Medeiros Marques¹  João Batista Buzato¹  Maria Antonia Pedrine Colabone Celligoi¹ 

[1] ,Universidade Estadual de Londrina Centro de Ciências Exatas Departamento de Bioquímica e BiotecnologiaLondrina Paraná ,Brasil

关键词: Immobilization;    invertase;    Luffa cylindrica;    raffinose;    Saccharomyces cerevisiae;    ultrasound;   

DOI  :  10.1590/S1516-89132006000700003

来源: SciELO

【 摘 要 】

This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These results showed that ultrasound application in continuous culture could have great potential for application in biotechnological techniques.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License