Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi

Brazilian Archives of Biology and Technology

Terezinha De Jesus Faria²  Rafael Sottero Ferreira²  Lidiane Yassumoto¹  José Roberto Pinto De Souza¹  Noemia Kazue Ishikawa¹  Aneli De Melo Barbosa¹ 

[1] ,Universidade Estadual de Londrina CCE Departamento de Química

关键词: Ocimum gratissimum;    antifungal activity;    eugenol;    phytopathogenic fungi;   

DOI  :  10.1590/S1516-89132006000700002

来源: SciELO

【 摘 要 】

An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied. The antifungal activity of eugenol was evaluated against a species of Alternaria isolated from tomato (A1) and Penicillium chrysogenum. The minimal inhibitory concentrations of eugenol were 0.16 and 0.31 mg/disc for Alternaria sp. (A1) and P. chrysogenum, respectively.

【 授权许可】

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