Brazilian Archives of Biology and Technology | |
Rice bran as a substrate for proteolytic enzyme production | |
Alagarsamy Sumantha2  Paul Deepa2  Chandran Sandhya2  George Szakacs1  Carlos Ricardo Soccol1  Ashok Pandey2  | |
[1] ,CSIR Regional Research Laboratory Biotechnology DivisionTrivandrum,India | |
关键词: Solid-state fermentation; Rice bran; Rhizopus; neutral protease; agro-industrial residues; | |
DOI : 10.1590/S1516-89132006000600019 | |
来源: SciELO | |
【 摘 要 】
Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30 C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme purity was achieved in this manner. The enzyme was found to be a metalloprotease, being activated by Mn2+, with maximal activity at a temperature of 60 C and pH 7.0.
【 授权许可】
CC BY-NC
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