Rice bran as a substrate for proteolytic enzyme production

Brazilian Archives of Biology and Technology

Alagarsamy Sumantha²  Paul Deepa²  Chandran Sandhya²  George Szakacs¹  Carlos Ricardo Soccol¹  Ashok Pandey² 

[1] ,CSIR Regional Research Laboratory Biotechnology DivisionTrivandrum,India

关键词: Solid-state fermentation;    Rice bran;    Rhizopus;    neutral protease;    agro-industrial residues;   

DOI  :  10.1590/S1516-89132006000600019

来源: SciELO

【 摘 要 】

Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30 C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme purity was achieved in this manner. The enzyme was found to be a metalloprotease, being activated by Mn2+, with maximal activity at a temperature of 60 C and pH 7.0.

【 授权许可】

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