Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system

Brazilian Archives of Biology and Technology

Raquel Pedrosa Bezerra²  Fernanda Katharine Sousa Lins Borba²  Keila Aparecida Moreira¹  José Luis Lima-filho¹  Ana Lúcia Figueiredo Porto¹  Adilson Castro Chaves² 

[1] ,Universidade de Pernambuco Instituto de Ciências Biológicas Recife PE ,Brasil

关键词: Amylases;    aqueous two-phase systems;    Bacillus subtilis;    partition;    poly (ethylene glycol);   

DOI  :  10.1590/S1516-89132006000500003

来源: SciELO

【 摘 要 】

Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.

【 授权许可】

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