Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

Brazilian Archives of Biology and Technology

Alessandra Ferreira Ribas²  Adilson Kenji Kobayashi¹  Luiz Filipe Protasio Pereira¹  Luiz Gonzaga Esteves Vieira² 

[1] ,Instituto Agronômico do Paraná Laboratório de Biotecnologia Vegetal Londrina PR ,Brasil

关键词: Genetic transformation;    coffee;    herbicide tolerance;    ammonium glufosinate;   

DOI  :  10.1590/S1516-89132006000100002

来源: SciELO

【 摘 要 】

Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl)-adenine (2-iP). Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L-1 of FinaleTM, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.

【 授权许可】

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