Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells

Brazilian Archives of Biology and Technology

Celso Aparecido Polinarski¹  José Luis Da Conceição Silva¹  Liya Regina Mikami¹  Maria Aparecida Fernandez¹ 

[1] ,Universidade Estadual de Maringá Departamento de Biologia Celular e Genética Maringá PR ,Brazil

关键词: Insect polytene cells;    Nuclear matrix;    Nuclear halo;    DNA loops;    Salivary gland;    Bradysia hygida;   

DOI  :  10.1590/S1516-89132005000300004

来源: SciELO

【 摘 要 】

A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License