Esterase polymorphism marking cultivars of Manihot esculenta, Crantz

Brazilian Archives of Biology and Technology

Adriana Gazoli Resende¹  Pedro Soares Vidigal Filho²  Maria De Fátima P. S. Machado¹ 

[1] ,Universidade Estadual de Maringá Departamento de Agronomia Maringá PR ,Brazil

关键词: Cassava;    isozymes;    esterases;    Manihot esculenta;    cultivar-specific isoesterases;   

DOI  :  10.1590/S1516-89132004000300003

来源: SciELO

【 摘 要 】

Esterase isozymes were used to detected substrate-preference polymorphism in twenty cultivars of Manihot esculenta, and to show cultivar-specific variation of this species. A relatively complex extraction solution of proteins from leaves was needed to show a larger number of esterase isozymes. Similarity between cultivars from six groups ranged from 51 to 96%. The cultivars identified by the same name seemed to be biochemically different regarding esterase isozymes. Esterase isozyme electrophoretic patterns could, therefore, be used to discriminate the cultivars identified by the same name, and to monitor the vegetative propagation of cultivars maintained in the germplasm collection. In breeding strategies, isoesterase analysis could be used to avoid intercrossing between the similar genotypes.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License