Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani

Brazilian Archives of Biology and Technology

Larissa Brandão Góes¹  Ana Bolena Lima Da Costa¹  Laurineide Lopes De Carvalho Freire¹  Neiva Tinti De Oliveira¹ 

[1] ,Universidade Federal de Pernambuco CCB Depto. MicologiaRecife PE ,Brasil

关键词: RAPD;    Trichoderma;    Rhizoctonia solani;   

DOI  :  10.1590/S1516-89132002000200005

来源: SciELO

【 摘 要 】

Random Amplified Polymorphic DNA (RAPD) procedure was used to examine the genetic variability among fourteen isolates of Trichoderma and their ability to antagonize Rhizoctonia solani using a dual-culture assay for correlation among RAPD products and their hardness to R. solani. Seven oligodeoxynucleotide primers were selected for the RAPD assays which resulted in 197 bands for 14 isolates of Trichoderma. The data were entered into a binary matrix and a similarity matrix was constructed using DICE similarity (SD) index. A UPGMA cluster based on SD values was generated using NTSYS (Numerical Taxonomy System, Applied Biostatistics) computer program. A mean coefficient of similarity obtained for pairwise comparisons among the most antagonics isolates was around 40%. The results presented here showed that the variability among the isolates of Trichoderma was very high. No relationship was found between the polymorphism showed by the isolates and their hardness, origin and substrata.

【 授权许可】

CC BY-NC   
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