Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)

Brazilian Archives of Biology and Technology

Itayguara Ribeiro Da Costa²  Petrônio Augusto Simão De Souza²  Carlos Bloch Jr.¹  Romulo Marino Llamoca-zárate²  Francisco A. P. Campos² 

[1] ,Universidade Federal do Ceará Departmento de Bioquímica e Biologia Molecular1 Fortaleza CE ,Brasil

关键词: Cereus jamacaru;    mandacarú;    Cactaceae;    seed proteins;    2S albumins;   

DOI  :  10.1590/S1516-89132001000400002

来源: SciELO

【 摘 要 】

We describe here the isolation and characterisation of a major reserve protein from the seeds of Cereus jamacaru. (Cactaceae). This protein has a molecular mass of 5319 kDa and was isolated by a combination of gel filtration chromatography and reverse phase HPLC. The amino acid composition of the protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The usefulness of this protein as a molecular marker in the Cactaceae is also discussed.

【 授权许可】

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