Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)

Brazilian Journal of Medical and Biological Research

T.c.f.r. Aragão²  P.a.s. Souza¹  A.f. Uchôa¹  I.r. Costa¹  C. Bloch Jr.¹  F.a.p. Campos² 

[1] ,Universidade Estadual do CearáFortaleza CE ,Brasil

关键词: Cactaceae;    mandacaru;    Cereus jamacaru;    cactin;    2S albumins;    methionine-rich protein;   

DOI  :  10.1590/S0100-879X2000000800005

来源: SciELO

【 摘 要 】

We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.

【 授权许可】

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