Purification of papain from fresh latex of Carica papaya

Brazilian Archives of Biology and Technology

Rubens Monti²  Carmelita A. Basilio²  Henrique C. Trevisan¹  Jonas Contiero¹ 

[1] ,Universidade Estadual Paulista Departamento de Alimentos e Nutrição Araraquara SP ,Brazil

关键词: Papain;    Carica papaya;    cysteine proteinase;    proteolytic enzymes;    purification of papain;   

DOI  :  10.1590/S1516-89132000000500009

来源: SciELO

【 摘 要 】

In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.

【 授权许可】

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