Culture medium for amylase production by toxigenic fungi

Brazilian Archives of Biology and Technology

Edson Luiz Zangrando Figueira¹  Elisa Yoko Hirooka¹ 

[1] ,Universidade Estadual de Londrina CCA Departamento de Tecnologia de Alimentos e MedicamentosLondrina PR ,Brasil

关键词: Amylase;    Fusarium moniliforme;    Aspergillus flavus;   

DOI  :  10.1590/S1516-89132000000500003

来源: SciELO

【 摘 要 】

Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day of fermentation, both for F. moniliforme (42.32U/mL) and A. flavus (4,745.54U/mL).

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License