Brazilian Archives of Biology and Technology | |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar | |
Julio Montañez Sáenz2  Alejandro Téllez1  Heliodoro De La Garza2  María De La Luz Reyes2  Juan Carlos Contreras-esquivel2  Cristóbal Noé Aguilar2  | |
[1] ,Universidad Autónoma de Coahuila Faculty of Chemical Sciences Food Research Dept.Saltillo Coah ,Mexico | |
关键词: Extraction; purification; properties; pectinesterase; potato; | |
DOI : 10.1590/S1516-89132000000400007 | |
来源: SciELO | |
【 摘 要 】
Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.
【 授权许可】
CC BY-NC
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