Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar

Brazilian Archives of Biology and Technology

Julio Montañez Sáenz²  Alejandro Téllez¹  Heliodoro De La Garza²  María De La Luz Reyes²  Juan Carlos Contreras-esquivel²  Cristóbal Noé Aguilar² 

[1] ,Universidad Autónoma de Coahuila Faculty of Chemical Sciences Food Research Dept.Saltillo Coah ,Mexico

关键词: Extraction;    purification;    properties;    pectinesterase;    potato;   

DOI  :  10.1590/S1516-89132000000400007

来源: SciELO

【 摘 要 】

Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.

【 授权许可】

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