Production of spores of Trichoderma harzianum on sugar cane molasses and bagasse pith in solid state fermentation for biocontrol

Brazilian Archives of Biology and Technology

Jose A. Rodríguez-león²  F. Domenech²  M. León²  T. Méndez²  D. E. Rodríguez²  Ashok Pandey¹ 

[1] ,Instituto Cubano de Investigaciones de los Derivados de la Caña de AzúcarLa Habana,Cuba

关键词: Trichoderma harzianum;    solid state fermentation;    sugar cane pith and molasses;    biocontrol;    kinetics;   

DOI  :  10.1590/S1516-89131999000100010

来源: SciELO

【 摘 要 】

Solid state fermentation was carried out for the production of spores from Trichoderma harzianum No 53 using sugar cane bagasse pith as solid matrix and sugar cane molasses as carbon and energy source. Different nitrogen sources such as urea, (NH4)2SO4 , NH4H2PO4 and (NH4)2HPO4 were added in the media to test their effect on spores production. Among these, urea was found most useful that resulted high no of spores (1x10(9)/gDM). The influence of temperature and initial moisture of the substrate was studied through a 2² experimental plan design. No statistical differences were found within the range of 30-35ºC and 60-70% for temperature and moisture respectively. The biotechnological parameters of the process were derived from the Oxygen Uptake Rate (OUR) pattern, which corresponded to the order of 10(9)spores/g moist material. The specific growth rate, maintenance coefficient and the yield based on O2 consumption were 0.108 h-1, 0.001 g.O2/g.biomass.h and 2.7 g biomass/g O2 consumed, respectively.

【 授权许可】

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