Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa

Revista Brasileira de Ciência Avícola

Gh De Oliveira¹  Â Berchieri Júnior¹  Hj Montassier¹  Ac Fernandes¹ 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia AnimalJaboticabal

关键词: Chickens;    conjugate;    ELISA;    Salmonella sp;    serology;   

DOI  :  10.1590/S1516-635X2004000200007

来源: SciELO

【 摘 要 】

This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.

【 授权许可】

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