Cold ischemia or topical-ECMO for lung preservation: a randomized experimental study

Sao Paulo Medical Journal

Alessandro Wasum Mariani¹  Israel Lopes Medeiros¹  Paulo Manuel Pego-fernandes¹  Flavio Guimaraes Fernandes¹  Fernando Do Vale Unterpertinguer¹  Lucas Matos Fernandes¹  Paulo Francisco Cardoso¹  Mauro Canzian¹  Fabio Biscegli Jatene¹ 

关键词: Organ preservation;    Reperfusion injury;    Lung transplantation;    Transplantation;    homologous;    Thoracic surgery;    Preservacao de orgaos;    Traumatismo por reperfusao;    Transplante de pulmao;    Transplante homologo;    Cirurgia toracica;   

DOI  :  10.1590/1516-3180.2014.1321594

来源: SciELO

【 摘 要 】

CONTEXT AND OBJECTIVE:Lung preservation remains a challenging issue for lung transplantation groups. Along with the development of ex vivo lung perfusion, a new preservation method known as topical-ECMO (extracorporal membrane oxygenation) has been proposed. The present study compared topical-ECMO with cold ischemia (CI) for lung preservation in an ex vivo experimental model.DESIGN AND SETTING:Randomized experimental study, conducted at a public medical school.METHOD:Fourteen human lungs were retrieved from seven brain-dead donors that were considered unsuitable for transplantation. The lung bloc was divided and each lung was randomized to be preserved by means of topical-ECMO or CI (4-7 °C) for eight hours. These lungs were then reconnected to an ex vivo perfusion system for functional evaluation. Lung biopsies were obtained at three times. The functional variables assessed were oxygenation capacity (OC) and pulmonary artery pressure (PAP); and the histological variables were lung injury score (LIS) and apoptotic cell count (ACC).RESULTS : The mean OC was 468 mmHg (± 81.6) in the topical-ECMO group and 455.8 (± 54) for CI (P = 0.758). The median PAP was 140 mmHg (120-160) in the topical-ECMO group and 140 mmHg (140-150) for CI (P = 0.285). The mean LIS was 35.57 (± 4.5) in the topical-ECMO group and 33.86 (± 6.1) for CI (P = 0.367). The ACC was 25.00 (± 9.34) in the topical-ECMO group and 24.86 (± 10.374) for CI (P = 0.803).CONCLUSIONS:The present study showed that topical-ECMO was not superior to cold ischemia for up to eight hours of lung preservation.

【 授权许可】

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