Qualitative and quantitative assessment of genetically modified soy in enteral nutrition formulas by polymerase chain reaction based methods

Revista de Nutrição

Natália Eudes Fagundes De Barros²  Edna Maria Morais Oliveira²  Otniel Freitas Silva²  Joab Trajano Silva¹  Vânia Margaret Flosi Paschoalin¹ 

[1] ,Embrapa Agroindústria de AlimentosRio de Janeiro RJ ,Brasil

关键词: Soy protein isolate;    Enteral nutrition;    Organisms genetically modified;    Food traceability;    Polymerase chain reaction;    Isolado protéico de soja;    Nutrição enteral;    Organismos geneticamente modificados;    Rastreabilidade alimentar;    Reação em cadeia da polimerase;   

DOI  :  10.1590/S1415-52732010000100006

来源: SciELO

【 摘 要 】

OBJECTIVE: The aim of this work was to investigate the occurrence of Roundup Ready soybean in enteral nutrition formulas sold in Brazil. METHODS: A duplex Polymerase Chain Reaction based on the amplification of the lectin gene and the construction of the recombinant deoxyribonucleic acid of transgenic glyphosate-tolerant soybean (35S promoter and chloroplast transit peptide gene) was performed in order to analyze the deoxyribonucleic acid obtained from nine soy protein isolate-containing formulas. RESULTS: Despite the highly processed nature of the food matrices, amplifiable deoxyribonucleic acid templates were obtained from all tested samples, as judged by the amplification of the lectin gene sequence. However, amplicons relative to the presence of Roundup Ready soybean were restricted to one of the nine enteral nutrition formulas analyzed as well as to the soybean reference powder, as expected. Quantitative analysis of the genetically modified formula by real-time Polymerase Chain Reaction showed a content of approximately 0.3% (w/w) of recombinant deoxyribonucleic acid from the Roundup Ready soybean. CONCLUSION: The results show that one of the formulas contained genetically modified soy, pointing to the need of regulating the use of transgenic substances and of specific labeling in this product category.

【 授权许可】

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