Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale

Genetics and Molecular Biology

Mariella Nieddu¹  Roberto Mezzanotte¹  Giuseppina Pichiri¹  Pier Paolo Coni¹  Gian Luca Dedola¹  Maria Luisa Dettori¹  Michele Pazzola¹  Giuseppe Massimo Vacca¹  Renato Robledo¹ 

关键词: Bovidae;    satellite DNA;    fluorescence in situ hybridization;    pericentromeric region;   

DOI  :  10.1590/S1415-475738420150094

来源: SciELO

【 摘 要 】

Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori.

【 授权许可】

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