Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)

Genetics and Molecular Biology

Monique Mantovani¹  Luciano Douglas Dos Santos Abel¹  Carlos Alberto Mestriner¹  Orlando Moreira-filho¹ 

[1] ,Universidade Federal de São Carlos Departamento de Genética e Evolução São Carlos SP ,Brazil

关键词: satellite DNA;    chromosome structure;    base-specific fluorochromes;    heterochromatin heterogeneity;    fluorescence in situ hybridization;   

DOI  :  10.1590/S1415-47572004000400012

来源: SciELO

【 摘 要 】

The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive heterochromatins. Such differences in chromosome banding patterns together with other karyotypic differences suggest differentiation of these populations at taxonomic level.

【 授权许可】

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